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Evaluation of a Multicolor, Single-Tube Technique To Enumerate Lymphocyte Subpopulations
Author(s) -
Federico Colombo,
Alessandra Cattaneo,
R. Lopa,
Paola Portararo,
Paolo Rebulla,
Laura Porretti
Publication year - 2008
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00481-07
Subject(s) - immunophenotyping , software , computer science , lymphocyte , immunology , biology , operating system , antigen
To evaluate the fully automated FACSCanto software, we compared lymphocyte subpopulation counts obtained using three-color FACSCalibur-CELLQuest and six-color FACSCanto-FACSCanto software techniques. High correlations were observed between data obtained with these techniques. Our study indicated that FACSCanto clinical software is accurate and sensitive in single-platform lymphocyte immunophenotyping.

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