Monocyte CD163 and CD36 Expression in Human Whole Blood and Isolated Mononuclear Cell Samples: Influence of Different Anticoagulants
Author(s) -
Marcin Moniuszko,
Krzysztof Kowal,
Małgorzata Rusak,
Mirosława Pietruczuk,
M Dąbrowska,
Anna BodzentaŁukaszyk
Publication year - 2006
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cvi.00417-05
Subject(s) - cd36 , cd163 , peripheral blood mononuclear cell , scavenger receptor , monocyte , receptor , biology , immunology , cell , macrophage , in vitro , biochemistry , cholesterol , lipoprotein
We investigated whether the choice of anticoagulant or the application of density gradient mononuclear cell isolation may account for conflicting published data regarding the levels of the scavenger receptors' expression in healthy individuals. We demonstrate that the detection of CD163, but not CD36, differs dramatically among the methods.
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