Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections | Zendy

Antonio Marcilla | Zendy; José Enrique De la Rubia | Zendy; Javier Sotillo | Zendy; Dolores Bernal | Zendy; Carlos Carmona | Zendy; Z. Villavicencio | Zendy; Daniel Acosta | Zendy; José F. Tort | Zendy; F. Bornay | Zendy; J. Guillermo Esteban | Zendy; Rafael Toledo | Zendy

Open Access

Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections

Author(s) -

Antonio Marcilla,

José Enrique De la Rubia,

Javier Sotillo,

Dolores Bernal,

Carlos Carmona,

Z. Villavicencio,

Daniel Acosta,

José F. Tort,

F. Bornay,

J. Guillermo Esteban,

Rafael Toledo

Publication year - 2007

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.00338-07

Subject(s) - fasciola hepatica , aminopeptidase , antigen , excretory system , biology , microbiology and biotechnology , leucine , biochemistry , immunology , helminths , amino acid , endocrinology

The liver fluke Fasciola hepatica parasitizes humans and ruminant livestock worldwide, and it is now being considered a reemerging zoonotic disease, especially in areas in which it is endemic, such as South America. This study investigates the immune response to excretory and secretory products produced by F. hepatica in a group of patients from the Peruvian Altiplano, where the disease is highly endemic. Using a proteomic approach and immunoblotting techniques, we have identified the enzymes leucine aminopeptidase (LAP) and phosphoenolpyruvate carboxykinase as immunodominant antigens recognized by sera from fasciolosis patients. An indirect enzyme-linked immunosorbent assay using recombinant LAP as the antigen was developed to check sera from individuals of this region. Our results demonstrate that LAP produces a specific and strong reaction, suggesting its potential use in the serologic diagnosis of F. hepatica infections in humans.

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