European Multicenter Evaluation of Commercial Enzyme Immunoassays for Detecting Norovirus Antigen in Fecal Samples | Zendy

Jim Gray | Zendy; Évelyne Kohli | Zendy; Franco Maria Ruggeri | Zendy; Harry Vennema | Zendy; Alicia Sánchez-Fauquier | Zendy; E. Schreier | Zendy; Chris I. Gallimore | Zendy; Miren IturrizaGómara | Zendy; H. Giraudon | Zendy; Pierre Pothier | Zendy; Ilaria Di Bartolo | Zendy; Nadia Inglese | Zendy; Erwin de Bruin | Zendy; Bas van der Veer | Zendy; Sílvia Moreno | Zendy; Vanessa Montero | Zendy; Marí C. de Llano | Zendy; Marina Höhne | Zendy; Sabine Diedrich | Zendy

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European Multicenter Evaluation of Commercial Enzyme Immunoassays for Detecting Norovirus Antigen in Fecal Samples

Author(s) -

Jim Gray,

Évelyne Kohli,

Franco Maria Ruggeri,

Harry Vennema,

Alicia Sánchez-Fauquier,

E. Schreier,

Chris I. Gallimore,

Miren IturrizaGómara,

H. Giraudon,

Pierre Pothier,

Ilaria Di Bartolo,

Nadia Inglese,

Erwin de Bruin,

Bas van der Veer,

Sílvia Moreno,

Vanessa Montero,

Marí C. de Llano,

Marina Höhne,

Sabine Diedrich

Publication year - 2007

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cvi.00214-07

Subject(s) - norovirus , feces , immunoassay , virology , multicenter study , antigen , microbiology and biotechnology , biology , antibody , immunology , medicine , virus , randomized controlled trial

A total of 2,254 fecal samples were tested in a European multicenter evaluation of commercially available norovirus antigen detection assays. Two commercial enzyme immunoassays, IDEIA Norovirus (Oxoid; Thermo Fisher Scientific, Ely, United Kingdom) and RIDASCREEN Norovirus (R-Biopharm, Darmstadt, Germany), were included in the evaluation, and their performance was compared with the results of reverse transcription-PCR (RT-PCR). Included in the evaluation were samples collected in sporadic cases of gastroenteritis, samples from outbreaks in which two or more samples were collected, well-characterized samples representing genotypes currently cocirculating within Europe, and samples collected from patients with gastroenteritis caused by a pathogen other than norovirus. The sensitivities and specificities of the IDEIA Norovirus and RIDASCREEN Norovirus assays were 58.93 and 43.81% and 93.91 and 96.37%, respectively, compared with RT-PCR. The sensitivities of both assays for outbreak investigations improved when six or more samples from an outbreak were examined. The IDEIA Norovirus assay exhibited reactivity to a broader range of norovirus genotypes than the RIDASCREEN Norovirus assay, which showed genotype-dependent sensitivities. The results indicate that, if used, these assays should serve as screening assays and the results should be confirmed by RT-PCR.

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