Effect of Contaminating Red Blood Cells on OKT3-Mediated Polyclonal Activation of Peripheral Blood Mononuclear Cells | Zendy

Samuel M. Song | Zendy; Joseph Goodwin | Zendy; Jenny Zhang | Zendy; Bruce Babbitt | Zendy; Janet L. Lathey | Zendy

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Effect of Contaminating Red Blood Cells on OKT3-Mediated Polyclonal Activation of Peripheral Blood Mononuclear Cells

Author(s) -

Samuel M. Song,

Joseph Goodwin,

Jenny Zhang,

Bruce Babbitt,

Janet L. Lathey

Publication year - 2002

Publication title -

clinical and vaccine immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.649

H-Index - 77

eISSN - 1556-6811

pISSN - 1556-679X

DOI - 10.1128/cdli.9.3.708-712.2002

Subject(s) - peripheral blood mononuclear cell , stimulation , chemistry , immunology , tumor necrosis factor alpha , red blood cell , cd8 , cytokine , polyclonal antibodies , interleukin 2 , il 2 receptor , interferon gamma , phorbol , microbiology and biotechnology , biology , immune system , endocrinology , t cell , in vitro , antigen , biochemistry , enzyme , protein kinase c

Erythrocytes are typically present as impurities in the majority of peripheral blood mononuclear cell (PBMC) preparations. This study was undertaken to investigate the effects of contaminating red blood cells (RBC) on the ability of OKT3 to activate CD4(+) and CD8(+) T cells. Surprisingly, the levels of gamma interferon, tumor necrosis factor alpha, and interleukin-1 beta (IL-1 beta) produced by PBMC upon stimulation by OKT3 were increased (P < 0.05) in a dose-dependent manner when increasing amounts of autologous RBC (RBC-to-PBMC ratios of 2:1, 10:1, and 50:1) were spiked into PBMC preparations. The OKT3-driven induction of the IL-2 receptor (CD25) and the proliferation of T lymphocytes in response to phorbol myristate acetate were not affected by the addition of RBC.

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