Rapid Detection of Salmonella enterica Serovar Choleraesuis in Blood Cultures by a Dot Blot Enzyme-Linked Immunosorbent Assay | Zendy

Kritsana Janyapoon | Zendy; Sunee Korbsrisate | Zendy; Hatairat Thamapa | Zendy; Sittichai Thongmin | Zendy; Suwattana Kanjanahareutai | Zendy; Niramol Wongpredee | Zendy; S Sarasombath | Zendy

Open Access

Rapid Detection of Salmonella enterica Serovar Choleraesuis in Blood Cultures by a Dot Blot Enzyme-Linked Immunosorbent Assay

Author(s) -

Kritsana Janyapoon,

Sunee Korbsrisate,

Hatairat Thamapa,

Sittichai Thongmin,

Suwattana Kanjanahareutai,

Niramol Wongpredee,

S Sarasombath

Publication year - 2000

Publication title -

clinical and diagnostic laboratory immunology

Language(s) - English

Resource type - Journals

eISSN - 1098-6588

pISSN - 1071-412X

DOI - 10.1128/cdli.7.6.977-979.2000

Subject(s) - serotype , salmonella enterica , salmonella , dot blot , microbiology and biotechnology , biology , monoclonal antibody , bacteria , western blot , virology , enterobacteriaceae , antibody , escherichia coli , immunology , biochemistry , gene , genetics

A dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella enterica serovar Choleraesuis in blood cultures. This system was applied to the identification of serovar Choleraesuis, and the results were compared with those obtained by a conventional biochemical method. It was revealed that all 12 samples identified to be infected with serovar Choleraesuis were positive on testing by the ELISA. In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%.

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