Viability and Functional Activity of Cryopreserved Mononuclear Cells | Zendy

Adriana Weinberg | Zendy; Li Zhang | Zendy; Darby Brown | Zendy; Alejo Erice | Zendy; Bruce Polsky | Zendy; Martin Hirsch | Zendy; Susan Owens | Zendy; Karan Lamb | Zendy

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Viability and Functional Activity of Cryopreserved Mononuclear Cells

Author(s) -

Adriana Weinberg,

Li Zhang,

Darby Brown,

Alejo Erice,

Bruce Polsky,

Martin Hirsch,

Susan Owens,

Karan Lamb

Publication year - 2000

Publication title -

clinical and diagnostic laboratory immunology

Language(s) - English

Resource type - Journals

eISSN - 1098-6588

pISSN - 1071-412X

DOI - 10.1128/cdli.7.4.714-716.2000

Subject(s) - peripheral blood mononuclear cell , cryopreservation , viability assay , andrology , immunology , lymphocyte , biology , medicine , cell , in vitro , microbiology and biotechnology , biochemistry , embryo

Factors that influence viability and function of cryopreserved peripheral blood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Clinical Trial Units. PBMC viability ranged from 1 to 96% with a median of 70%, was higher in laboratories with experienced staff, and was not significantly associated with CD4 cell number. Function of cryopreserved PBMC, measured by lymphocyte proliferation, was associated with viability. Preparations with viability greater than or equal to 70% had consistent proliferative responses and were suitable for functional analyses.

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