Development of a Recombinant Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay for Quantification of Antibodies against Porcine Reproductive and Respiratory Syndrome Virus | Zendy

Steven B. Witte | Zendy; Cindy ChardBergstrom | Zendy; Thomas A. Loughin | Zendy; Sanjay Kapil | Zendy

Open Access

Development of a Recombinant Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay for Quantification of Antibodies against Porcine Reproductive and Respiratory Syndrome Virus

Author(s) -

Steven B. Witte,

Cindy ChardBergstrom,

Thomas A. Loughin,

Sanjay Kapil

Publication year - 2000

Publication title -

clinical and diagnostic laboratory immunology

Language(s) - English

Resource type - Journals

eISSN - 1098-6588

pISSN - 1071-412X

DOI - 10.1128/cdli.7.4.700-702.2000

Subject(s) - porcine reproductive and respiratory syndrome virus , nucleoprotein , recombinant dna , virology , antibody , virus , enzyme , biology , respiratory system , microbiology and biotechnology , immunology , biochemistry , gene , anatomy

A rapid, inexpensive enzyme-linked immunosorbent assay (ELISA) to quantitate antibodies to porcine respiratory and reproductive syndrome virus (PRRSV) in serum was developed using a recombinant PRRSV nucleoprotein (rN). The sensitivity (85.3%) and specificity (81.7%) of the Kansas State University ELISA were good, correlating well (82.4%) with the IDEXX HerdChek ELISA.

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