Open AccessComparison of Microcapillary Cytometry Technology and Flow Cytometry for CD4 + and CD8 + T-Cell Estimation
Author(s) -
AJ Kandathil,
Rajesh Kannangai,
S. David,
G Nithyanandam,
Suniti Solomon,
Pachamuthu Balakrishnan,
O C Abraham,
S. Subramanian,
Priscilla Rupali,
V. P. Verghese,
SA Pulimood,
Gopalan Sridharan
Publication year - 2005
Publication title -
clinical and vaccine immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.649
H-Index - 77
eISSN - 1556-6811
pISSN - 1556-679X
DOI - 10.1128/cdli.12.8.1006-1009.2005
Subject(s) - flow cytometry , cd8 , cytometry , correlation coefficient , coefficient of variation , human immunodeficiency virus (hiv) , microbiology and biotechnology , biology , chemistry , immunology , mathematics , statistics , chromatography , antigen
An alternative technology for the estimation of T cells based on a microcapillary technique (Guava Technologies, Hayward, CA) was compared to FACSCount (Becton Dickinson, San Jose, CA). Samples from 51 human immunodeficiency virus-infected and 21 healthy individuals were tested. The correlation (r) of the two systems for CD4(+) T cells was 0.994, and the coefficient of variation was 6.5%, establishing equable performance between the two technologies.