Salmonella-specific monoclonal antibodies against recombinant Salmonella typhi 36-kilodalton porin | Zendy

Vincent Kissel | Zendy; Carlos González | Zendy; Michael G. Astudillo | Zendy; Anne Godard | Zendy; B Wachman | Zendy; Felipe C. Cabello | Zendy

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Salmonella-specific monoclonal antibodies against recombinant Salmonella typhi 36-kilodalton porin

Author(s) -

Vincent Kissel,

Carlos González,

Michael G. Astudillo,

Anne Godard,

B Wachman,

Felipe C. Cabello

Publication year - 1994

Publication title -

clinical and diagnostic laboratory immunology

Language(s) - English

Resource type - Journals

eISSN - 1098-6588

pISSN - 1071-412X

DOI - 10.1128/cdli.1.2.250-252.1994

Subject(s) - monoclonal antibody , salmonella typhi , porin , salmonella , recombinant dna , biology , microbiology and biotechnology , antibody , virology , bacterial outer membrane , western blot , bacteria , escherichia coli , biochemistry , immunology , gene , genetics

Mouse monoclonal antibodies were raised against recombinant Salmonella typhi 36-kDa porin monomer. Specificities of 16 monoclonal antibodies were analyzed as reactivity patterns in dot immunobinding and Western blot (immunoblot) assays using isolated outer membrane proteins of gram-negative bacteria and cloned purified S. typhi porin monomers and trimers. Four monoclonal antibodies were specific for Salmonella spp.

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