Open AccessTransformation of Rhizobia with Broad-Host-Range Plasmids by Using a Freeze-Thaw Method
Author(s) -
Éva Vincze,
Steve Bowra
Publication year - 2006
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.72.3.2290-2293.2006
Subject(s) - rhizobia , plasmid , transformation (genetics) , replicon , biology , host (biology) , rhizobiaceae , range (aeronautics) , genetics , bacteria , symbiosis , gene , materials science , composite material
Several species of rhizobia were successfully transformed with broad-host-range plasmids of different replicons by using a modified freeze-thaw method. A generic binary vector (pPZP211) was maintained inMesorhizobium loti without selection and stably inherited during nodulation. The method could extend the potential of rhizobia as a vehicle for plant transformation.