Freezing Induces Biased Results in the Molecular Detection of Flavobacterium columnare | Zendy

Lotta-Riina Suomalainen | Zendy; Hilkka Reunanen | Zendy; Ritva Ijäs | Zendy; E. Tellervo Valtonen | Zendy; Marja Tiirola | Zendy

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Freezing Induces Biased Results in the Molecular Detection of Flavobacterium columnare

Author(s) -

Lotta-Riina Suomalainen,

Hilkka Reunanen,

Ritva Ijäs,

E. Tellervo Valtonen,

Marja Tiirola

Publication year - 2006

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.72.2.1702-1704.2006

Subject(s) - pathogen , biology , microbiology and biotechnology , flavobacterium , bacteria , fish <actinopterygii> , polymerase chain reaction , gene , genetics , pseudomonas , fishery

Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.

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