Probing In Vivo Promoter Activities in Mycoplasma pneumoniae : a System for Generation of Single-Copy Reporter Constructs | Zendy

Sven Halbedel | Zendy; Jörg Stülke | Zendy

Open Access

Probing In Vivo Promoter Activities in Mycoplasma pneumoniae : a System for Generation of Single-Copy Reporter Constructs

Author(s) -

Sven Halbedel,

Jörg Stülke

Publication year - 2006

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.72.2.1696-1699.2006

Subject(s) - mycoplasma pneumoniae , in vivo , biology , reporter gene , computational biology , genetics , microbiology and biotechnology , gene , medicine , gene expression , pneumonia

The nucleotide sequences that control transcription initiation and regulation inMycoplasma pneumoniae are poorly understood. In this work, we developed alacZ -based reporter plasmid that can be used to integrate fusions of promoter fragments to a promoterlesslacZ gene into the chromosome ofM. pneumoniae .

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