Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases | Zendy

Jan B. van Beilen | Zendy; Enrico G. Funhoff | Zendy; Alexander van Loon | Zendy; Andrea Just | Zendy; Leo Kaysser | Zendy; Manuel Bouza | Zendy; René Holtackers | Zendy; Martina Röthlisberger | Zendy; Zhi Li | Zendy; Bernard Witholt | Zendy

Open Access

Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases

Author(s) -

Jan B. van Beilen,

Enrico G. Funhoff,

Alexander van Loon,

Andrea Just,

Leo Kaysser,

Manuel Bouza,

René Holtackers,

Martina Röthlisberger,

Zhi Li,

Bernard Witholt

Publication year - 2006

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.72.1.59-65.2006

Subject(s) - alkane , hydroxylation , ferredoxin , cytochrome p450 , cytochrome , biology , biochemistry , gene , enzyme , pseudomonas putida , monooxygenase , chemistry , catalysis

Several strains that grow on medium-chain-length alkanes and catalyze interesting hydroxylation and epoxidation reactions do not possess integral membrane nonheme iron alkane hydroxylases. Using PCR, we show that most of these strains possess enzymes related to CYP153A1 and CYP153A6, cytochrome P450 enzymes that were characterized as alkane hydroxylases. A vector for the polycistronic coexpression of individual CYP153 genes with a ferredoxin gene and a ferredoxin reductase gene was constructed. Seven of the 11 CYP153 genes tested allowed Pseudomonas putida GPo12 recombinants to grow well on alkanes, providing evidence that the newly cloned P450s are indeed alkane hydroxylases.

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