Open AccessGenetic Loci of Major Antigenic Protein Genes ofEdwardsiella tarda
Author(s) -
Noel Verján,
Ikuo Hirono,
Takashi Aoki
Publication year - 2005
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.71.9.5654-5658.2005
Subject(s) - edwardsiella tarda , periplasmic space , biology , antiserum , polyclonal antibodies , immunogenicity , gene , recombinant dna , antigen , microbiology and biotechnology , protein subunit , flounder , epitope , genetics , escherichia coli , fish <actinopterygii> , fishery
Seven antigenic proteins of Edwardsiella tarda were identified by using a rabbit polyclonal antiserum. Four of these proteins also reacted with a Japanese flounder antiserum. The amino acid sequences had identity to lipoproteins, periplasmic proteins, and exported and secreted proteins with roles in transport of metabolites across the cell membrane, stress response, and motility. These genes and their products are useful for developing DNA or recombinant subunit vaccines to control edwardsiellosis.