Open AccessCharacterization of a Multicopper Oxidase Gene from Staphylococcus aureus
Author(s) -
Sutthirat Sitthisak,
K. Howieson,
C. Amezola,
Radheshyam K. Jayaswal
Publication year - 2005
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.71.9.5650-5653.2005
Subject(s) - staphylococcus aureus , multicopper oxidase , gene , microbiology and biotechnology , biology , genetics , computational biology , chemistry , bacteria , enzyme , biochemistry , laccase
A multicopper oxidase gene from Staphylococcus aureus was cloned and overexpressed. Purified recombinant multicopper oxidase oxidized the substrate 3,3'-dimethoxybenzidine in the presence of copper. Disruption of mco showed copper sensitivity and H(2)O(2) resistance, suggesting roles for mco in copper homeostasis and oxidative stress response. Northern blot analysis showed copper-induced mco transcription.