Open AccessGenomic DNA Amplification from a Single Bacterium
Author(s) -
Arumugham Raghunathan,
Harley R. Ferguson,
Carole J. Bornarth,
Wanmin Song,
Mark D. Driscoll,
Roger S. Lasken
Publication year - 2005
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.71.6.3342-3347.2005
Subject(s) - multiple displacement amplification , genomic dna , polymerase chain reaction , biology , dna , bacteria , genetics , 16s ribosomal rna , gene , dna polymerase , microbiology and biotechnology , dna extraction
Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.