Open AccessDifferential Real-Time PCR Assay for Enumeration of Lactic Acid Bacteria in Wine
Author(s) -
Ezekiel T. Neeley,
Trevor G. Phister,
David A. Mills
Publication year - 2005
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.71.12.8954-8957.2005
Subject(s) - oenococcus oeni , malolactic fermentation , wine , lactic acid , food spoilage , bacteria , wine fault , enumeration , food science , biology , fermentation , fermentation in winemaking , microbiology and biotechnology , genetics , mathematics , combinatorics
Oenococcus oeni is often employed to perform the malolactic fermentation in wine production, while nonoenococcal lactic acid bacteria often contribute to wine spoilage. Two real-time PCR assays were developed to enumerate the total, and nonoenococcal, lactic acid bacterial populations in wine. Used together, these assays can assess the spoilage risk of juice or wine from lactic acid bacteria.