Open AccessNoninvasive Pigment Identification in Single Cells from Living Phototrophic Biofilms by Confocal Imaging Spectrofluorometry
Author(s) -
Mónica Roldán,
Federico Thomas,
Susanna Castel,
Antonio Quesada,
Mariona Hernández-Mariné
Publication year - 2004
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.70.6.3745-3750.2004
Subject(s) - confocal , confocal laser scanning microscopy , confocal microscopy , pigment , fluorescence , biofilm , microscopy , fluorescence microscope , phototroph , fluorescence lifetime imaging microscopy , biophysics , chemistry , biology , optics , bacteria , microbiology and biotechnology , physics , genetics , organic chemistry
A new imaging technique for the analysis of fluorescent pigments from a single cell is reported. It is based on confocal scanning laser microscopy coupled with spectrofluorometric methods. The setup allows simultaneous establishment of the relationships among pigment analysis in vivo, morphology, and three-dimensional localization inside thick intact microbial assemblages.