Open AccessEstablishment of a Pure Culture of the Hitherto Uncultured Unicellular Cyanobacterium Aphanothece sacrum , and Phylogenetic Position of the Organism
Author(s) -
Takashi Fujishiro,
Takahira Ogawa,
Masayoshi Matsuoka,
Kazuhiro Nagahama,
Yasunobu Takeshima,
Hideaki Hagiwara
Publication year - 2004
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.70.6.3338-3345.2004
Subject(s) - sacrum , cyanobacteria , biology , strain (injury) , phylogenetic tree , 16s ribosomal rna , gene , botany , genetics , bacteria , anatomy
Aphanothece sacrum, an edible freshwater unicellular cyanobacterium, was isolated by using novel synthetic media (designated AST and AST-5xNP). The media were designed on the basis of the ratio of inorganic elements contained in A. sacrum cells cultured in a natural pond. The isolated strain exhibits unicellular rod-shaped cells approximately 6 microm in length that are scattered in an exopolysaccharide matrix, a feature similar to that of natural A. sacrum. DNA analysis of the isolated strain revealed that it carried two ferredoxin genes whose deduced amino acid sequences were almost identical to previously published sequences of ferredoxins from natural A. sacrum. Analysis of the 16S rRNA gene and ferredoxin genes revealed that A. sacrum occupies a phylogenetically unique position among the cyanobacteria.