Open AccessSelection of Single-Chain Antibodies against the VP8* Subunit of Rotavirus VP4 Outer Capsid Protein and Their Expression inLactobacillus casei
Author(s) -
Vicente Monedero,
Jesús Rodrı́guez-Dı́az,
Rosa Viana,
Javier Buesa,
Gaspar PérezMartínez
Publication year - 2004
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.70.11.6936-6939.2004
Subject(s) - capsid , rotavirus , protein subunit , lactobacillus casei , biology , antibody , microbiology and biotechnology , virology , selection (genetic algorithm) , reoviridae , virus , bacteria , genetics , gene , artificial intelligence , computer science
Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.