Open AccessDetection of Staphylococcal Enterotoxin B via Biomolecular Interaction Analysis Mass Spectrometry
Author(s) -
Dobrin Nedelkov,
Randall W. Nelson
Publication year - 2003
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.69.9.5212-5215.2003
Subject(s) - mass spectrometry , detection limit , chemistry , chromatography , surface plasmon resonance , enterotoxin , analyte , immunoassay , matrix assisted laser desorption/ionization , analytical chemistry (journal) , desorption , materials science , escherichia coli , nanotechnology , nanoparticle , biology , antibody , biochemistry , organic chemistry , adsorption , immunology , gene
Detection of Staphylococcus enterotoxin B (SEB) by biomolecular interaction analysis mass spectrometry (BIA/MS) is presented in this work. The BIA/MS experiments were based on a surface plasmon resonance (SPR) MS immunoassay that detects affinity-captured SEB both via SPR and by means of exact and direct mass measurement by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Experiments were performed with standard samples and food samples to assess the BIA/MS limit of detection for SEB and to set the experimental parameters for proper quantitation. Single and double SPR referencing was performed to accurately estimate the amount of the bound toxin. Reproducible detection of 1 ng of SEB per ml, corresponding to affinity capture and MS analysis of approximately 500 amol of SEB, was readily achieved from both the standard and mushroom samples. A certain amount of SEB degradation was indicated by the signals in the mass spectra. The combination of MS with SPR-based methods of detection creates a unique approach capable of quantifying and qualitatively analyzing protein toxins from pathogenic organisms.