Open AccessDetection of Helicobacter Colonization of the Murine Lower Bowel by Genus-Specific PCR-Denaturing Gradient Gel Electrophoresis
Author(s) -
Martin Grehan,
Gauri Tamotia,
Bronwyn R. Robertson,
Hazel M. Mitchell
Publication year - 2002
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.68.10.5164-5166.2002
Subject(s) - temperature gradient gel electrophoresis , biology , helicobacter , gel electrophoresis , polymerase chain reaction , microbiology and biotechnology , colonization , electrophoresis , bacteria , helicobacter pylori , 16s ribosomal rna , genetics , gene
Helicobacter genus-specific PCR and denaturing gradient gel electrophoresis can detect and speciate the helicobacters that colonize the lower bowel of laboratory mice. The method's sensitivity is comparable to that of species-specific PCR and may detect unnamed Helicobacter species. This approach should prove useful for commercial and research murine facilities.