Open AccessDouble-Staining Method for Differentiation of Morphological Changes and Membrane Integrity of Campylobacter coli Cells
Author(s) -
J.L. Alonso,
Salvatore Mascellaro,
Yolanda Moreno,
María Antonia Ferrús,
Javier Hernández
Publication year - 2002
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.68.10.5151-5154.2002
Subject(s) - staining , biology , stain , vital stain , campylobacter , membrane integrity , microbiology and biotechnology , membrane , bacteria , biochemistry , genetics
We developed a double-staining procedure involving NanoOrange dye (Molecular Probes, Eugene, Oreg.) and membrane integrity stains (LIVE/DEAD BacLight kit; Molecular Probes) to show the morphological and membrane integrity changes of Campylobacter coli cells during growth. The conversion from a spiral to a coccoid morphology via intermediary forms and the membrane integrity changes of the C. coli cells can be detected with the double-staining procedure. Our data indicate that young or actively growing cells are mainly spiral shaped (green-stained cells), but older cells undergo a degenerative change to coccoid forms (red-stained cells). Club-shaped transition cell forms were observed with NanoOrange stain. Chlorinated drinking water affected the viability but not the morphology of C. coli cells.