Open AccessDehalogenation of Chlorinated Hydroxybiphenyls by Fungal Laccase
Author(s) -
Asgard Schultz,
Ulrike Jonas,
Elke Hammer,
Frieder Schauer
Publication year - 2001
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.67.9.4377-4381.2001
Subject(s) - laccase , halogenation , chemistry , biotransformation , organic chemistry , mass spectrometry , gas chromatography , combinatorial chemistry , chromatography , enzyme
We have investigated the transformation of chlorinated hydroxybiphenyls by laccase produced by Pycnoporus cinnabarinus. The compounds used were transformed to sparingly water-soluble colored precipitates which were identified by gas chromatography-mass spectrometry as oligomerization products of the chlorinated hydroxybiphenyls. During oligomerization of 2-hydroxy-5-chlorobiphenyl and 3-chloro-4-hydroxybiphenyl, dechlorinated C---C-linked dimers were formed, demonstrating the dehalogenation ability of laccase. In addition to these nonhalogenated dimers, both monohalogenated and dihalogenated dimers were identified.