Open AccessDecrease in Cryptosporidium parvum Oocyst Infectivity In Vitro by Using the Membrane Filter Dissolution Method for Recovering Oocysts from Water Samples
Author(s) -
Ramón A. Carreño,
Nicholas J. Pokorny,
Susan C. Weir,
Hung Lee,
J. T. Trevors
Publication year - 2001
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.67.7.3309-3313.2001
Subject(s) - infectivity , cryptosporidium parvum , microbiology and biotechnology , membrane filter , filtration (mathematics) , cellulose acetate , acetone , in vitro , biology , dissolution , chromatography , cryptosporidium , membrane , chemistry , virology , biochemistry , feces , virus , statistics , mathematics
Exposure of Cryptosporidium parvum oocysts to solutions used for cellulose acetate membrane (CAM) dissolution filtration reduced their infectivity in HCT-8 cells. Ethanol (95% [vol/vol] and 70% [vol/vol]) alone and short exposure times to acetone decreased infectivity. These findings contrast with similar experiments using excystation assays and infectivity in mice.