Open AccessApplication of Rapid Dot Blot Immunoassay for Detection of Salmonella enterica Serovar Enteritidis in Eggs, Poultry, and Other Foods
Author(s) -
Mark Akira Yoshimasu,
Jerzy Zawistowski
Publication year - 2001
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.67.1.459-461.2001
Subject(s) - salmonella enteritidis , serotype , immunoassay , salmonella enterica , dot blot , salmonella , microbiology and biotechnology , biology , monoclonal antibody , inoculation , bacteria , western blot , virology , antibody , immunology , genetics , dna , biochemistry , gene
Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Serovar Enteritidis was detected in the presence of other bacteria when outcompeted 1:400.
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