Open AccessCloning, Nucleotide Sequence, and Expression of the Gene Encoding the Bacteriocin Boticin B from Clostridium botulinum Strain 213B
Author(s) -
Sean S. Dineen,
Marite Bradshaw,
Eric A. Johnson
Publication year - 2000
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.66.12.5480-5483.2000
Subject(s) - clostridium botulinum , bacteriocin , plasmid , biology , nucleic acid sequence , strain (injury) , gene , clostridium , shuttle vector , clostridiaceae , open reading frame , clostridia , sequence analysis , molecular cloning , cloning (programming) , genetics , microbiology and biotechnology , peptide sequence , recombinant dna , bacteria , vector (molecular biology) , toxin , anatomy , computer science , programming language
Boticin B is a heat-stable bacteriocin produced byClostridium botulinum strain 213B that has inhibitory activity against various strains ofC. botulinum and related clostridia. The gene encoding the bacteriocin was localized to a 3.0-kbHin dIII fragment of an 18.8-kb plasmid, cloned, and sequenced. DNA sequencing revealed the boticin B structural gene,btcB , to be an open reading frame encoding 50 amino acids. AC. botulinum strain 62A transconjugant containing theHin dIII fragment inserted into a clostridial shuttle vector expressed boticin B, although at much lower levels than those observed inC. botulinum 213B. To our knowledge, this is the first demonstration and characterization of a bacteriocin from toxigenic group IC. botulinum .