Open AccessColonization of the Stratified Squamous Epithelium of the Nonsecreting Area of Horse Stomach by Lactobacilli
Author(s) -
Norikatsu Yuki,
Tomoko Shimazaki,
Akira Kushiro,
Koichi Watanabe,
Kazumi Uchida,
Teruhiko Yuyama,
Masami Morotomi
Publication year - 2000
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.66.11.5030-5034.2000
Subject(s) - biology , stratified squamous epithelium , microbiology and biotechnology , lactobacillus salivarius , epithelium , stomach , bacteria , lactobacillus reuteri , colonization , horse , lactobacillus , genetics , biochemistry , paleontology
Selective adhesion to only certain epithelia is particularly common among the bacterial members of the indigenous microflora of mammals. We have found that the stratified squamous epithelium of the nonsecreting area of horse stomach is colonized by gram-positive rods. The microscopic features of a dense layer of these bacteria on the epithelium were found to be similar to those reported in mice, rats, and swine. Adhering microorganisms were isolated and identified asLactobacillus salivarius ,L. crispatus ,L. reuteri , andL. agilis by DNA-DNA hybridization and 16S rRNA gene sequencing techniques. These lactobacilli associated with the horse, except forL. reuteri , were found to adhere to horse epithelial cells in vitro but not to those of rats. A symbiotic relationship of these lactobacilli with the horse is suggested.