Characterization of the Cricket Hindgut Microbiota with Fluorescently Labeled rRNA-Targeted Oligonucleotide Probes | Zendy

Jorge W. Santo Domingo | Zendy; Michael G. Kaufman | Zendy; Michael J. Klug | Zendy; James M. Tiedje | Zendy

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Characterization of the Cricket Hindgut Microbiota with Fluorescently Labeled rRNA-Targeted Oligonucleotide Probes

Author(s) -

Jorge W. Santo Domingo,

Michael G. Kaufman,

Michael J. Klug,

James M. Tiedje

Publication year - 1998

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.64.2.752-755.1998

Subject(s) - hindgut , oligomer restriction , biology , microbiology and biotechnology , 16s ribosomal rna , ribosomal rna , prevotella , microorganism , bacteria , bacteroides , acheta , oligonucleotide , cricket , biochemistry , zoology , ecology , genetics , gene , larva , midgut

Most cricket hindgut microorganisms (60 to 80%) were detected with a universal fluorescent rRNA-targeted probe and found to be eubacteria. Group-specific probes showed that the hindguts of five different cricket species harbor similar bacterial groups, although in different proportions, and that different diets shifted the structure of the hindgut microbial community. TheBacteroides-Prevotella probe, of the eight eubacterial probes tested, stained the largest percentage of cells in all crickets.

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