Genetic Construction of Lactose-Utilizing Xanthomonas campestris | Zendy

Patricia M. Walsh | Zendy; Michael J. Haas | Zendy; George A. Somkuti | Zendy

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Genetic Construction of Lactose-Utilizing Xanthomonas campestris

Author(s) -

Patricia M. Walsh,

Michael J. Haas,

George A. Somkuti

Publication year - 1984

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.47.2.253-257.1984

Subject(s) - xanthomonas campestris , lactose , transposable element , xanthan gum , plasmid , nalidixic acid , microbiology and biotechnology , biology , pseudomonadaceae , pseudomonas aeruginosa , bacteria , chemistry , biochemistry , gene , escherichia coli , genetics , mutant , materials science , rheology , composite material

Xanthomonas campestris , the producer of xanthan gum, possesses a β-galactosidase of very low specific activity. Plasmid pGC9114 (RP1::Tn951 ), generated by the transposition of the lactose transposon Tn951 to RP1, was conjugally transferred into XN1, a nalidixic acid-resistant derivative ofX. campestris NRRL B-1459S-4L. Transfer occurred on membrane filters and in broth. The β-galactosidase gene of Tn951 was expressed inX. campestris . The specific activity of β-galactosidase in transconjugants was over 200-fold higher than that in XN1, and transconjugants grew as well in lactose-based media as in glucose-based media. The lactose-utilizing transconjugants could potentially be used to produce xanthan gum from cheese whey.

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