Replica Plating Method for Estimating Phenanthrene-Utilizing and Phenanthrene-Cometabolizing Microorganisms | Zendy

Michael P. Shiaris | Zendy; J. J. Cooney | Zendy

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Replica Plating Method for Estimating Phenanthrene-Utilizing and Phenanthrene-Cometabolizing Microorganisms

Author(s) -

Michael P. Shiaris,

J. J. Cooney

Publication year - 1983

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.45.2.706-710.1983

Subject(s) - phenanthrene , microorganism , environmental science , replica , environmental chemistry , chemistry , biology , bacteria , art , visual arts , genetics

A replica plating method was developed for detecting and enumerating phenanthrene-degrading microorganisms. The method is designed to discriminate between aquatic organisms that utilize phenanthrene as the sole carbon and energy source and organisms that cometabolize phenanthrene. The method was used to demonstrate that phenanthrene utilizers and phenanthrene cometabolizers coexist in estuarine sediments.

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