Open AccessUsefulness of Electrophoretic Pattern of Cell Envelope Protein as a Taxonomic Tool for Fishhold Slime Moraxella Species
Author(s) -
T J Chai
Publication year - 1981
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.42.2.351-356.1981
Subject(s) - cell envelope , moraxella , biology , incubation , polyacrylamide gel electrophoresis , envelope (radar) , electrophoresis , gel electrophoresis , sodium dodecyl sulfate , microbiology and biotechnology , sodium , bacteria , biochemistry , chemistry , escherichia coli , genetics , enzyme , gene , computer science , organic chemistry , radar , telecommunications
Nine independentMoraxella cultures were isolated from the accumulated slime in fishholds of fishery trawlers. It is significant that none of these isolates was viable above 30°C, a temperature well below the usual incubation temperature for plate counts of food samples. The traditional taxonomic parameters showed no significant dissimilarities among these closely related marine organisms or between them and conventional moraxellas. However, cell envelope protein profiles examined on sodium dodecyl sulfate-polyacrylamide gels revealed that the organisms fell into several distinct groups. The cell envelope protein profile could be a simple and quick test to determine the fine relationships between individual isolates.
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