Open AccessEnzyme Thermistor Analysis of Penicillin in Standard Solutions and in Fermentation Broth
Author(s) -
Bo Mattìasson,
Bengt Danielsson,
Fredrik Winquist,
H. Nilsson,
Klaus Mosbach
Publication year - 1981
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.41.4.903-908.1981
Subject(s) - thermistor , fermentation , chromatography , penicillin , dilution , chemistry , serial dilution , correlation coefficient , porous glass , biochemistry , porosity , antibiotics , mathematics , pathology , thermodynamics , organic chemistry , medicine , electrical engineering , physics , engineering , alternative medicine , statistics
Immobilized penicillinase was applied in an enzyme thermistor for calorimetric analysis of samples containing penicillin G. Standard solutions as well as extracts from fermentation broth were analyzed. The enzyme was applied bound either to porous glass or, when dealing with crude preparations, to the inner surface of nylon tubing. In the fermentation system studied, high concentrations of penicillin were present, thus allowing dilution to reduce the influence of the composition of the medium on the analysis. The useful linear concentration range was from 0.1 to 100 mM. The coefficient of correlation between analytical results obtained with the present method and those from conventional assays was 0.997.
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