Production and characterization of aflatoxin B2a antiserum | Zendy

Praveen Kumar Gaur | Zendy; H P Lau | Zendy; James J. Pestka | Zendy; F S Chu | Zendy

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Production and characterization of aflatoxin B2a antiserum

Author(s) -

Praveen Kumar Gaur,

H P Lau,

James J. Pestka,

F S Chu

Publication year - 1981

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.41.2.478-482.1981

Subject(s) - aflatoxin , antiserum , radioimmunoassay , bovine serum albumin , horseradish peroxidase , serum albumin , titer , chemistry , biochemistry , biology , microbiology and biotechnology , antibody , enzyme , food science , immunology

The specificity and sensitivity of antiserum elicited from rabbits against aflatoxin B2a-bovine serum albumin conjugates were characterized with a radioimmunoassay (RIA) and an enzyme-linked immunosorbent assay (ELISA). Aflatoxin B1 was first converted to aflatoxin B2a and then conjugated to bovine serum albumin and horseradish peroxidase by a reductive alkylation method. The antiserum was developed in New Zealand white rabbits by multiple-site injection with the aflatoxin B2a-bovine serum albumin conjugate. Antibody titers were determined by both RIA and ELISA. Competitive RIAs with various aflatoxin analogs indicated that the antiserum was most reactive with aflatoxin B1 and slightly cross-reactive with aflatoxins B2a, B2, and M1. Competitive ELISAs showed the antiserum to be equally specific for aflatoxins B2a and B12 and less reactive with aflatoxins B2 and M1. The relative sensitivities of RIA and ELISA for aflatoxin B1 quantitation were 100 and 10 pg per assay, respectively.

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