Community Structure Analysis of Methanogens Associated with Rumen Protozoa Reveals Bias in Universal Archaeal Primers | Zendy

Lisa Tymensen | Zendy; Tim A. McAllister | Zendy

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Community Structure Analysis of Methanogens Associated with Rumen Protozoa Reveals Bias in Universal Archaeal Primers

Author(s) -

Lisa Tymensen,

Tim A. McAllister

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.07994-11

Subject(s) - biology , rumen , archaea , protozoa , ribosomal rna , euryarchaeota , primer (cosmetics) , microbiology and biotechnology , metagenomics , methanogen , bacteria , genetics , gene , biochemistry , chemistry , organic chemistry , fermentation

The diversity of protozoan-associated methanogens in cattle was investigated using five universal archaeal small-subunit (SSU) rRNA gene primer sets. Methanobrevibacter spp. and rumen cluster C (distantly related to Thermoplasma spp.) were predominant. Significant differences in species composition among libraries indicate that some primers used previously to characterize rumen methanogens exhibit biased amplification.

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