Application of pheB as a Reporter Gene for Geobacillus spp., Enabling Qualitative Colony Screening and Quantitative Analysis of Promoter Strength | Zendy

Jeremy Bartosiak-Jentys | Zendy; Kirstin L. Eley | Zendy; David J. Leak | Zendy

Open Access

Application of pheB as a Reporter Gene for Geobacillus spp., Enabling Qualitative Colony Screening and Quantitative Analysis of Promoter Strength

Author(s) -

Jeremy Bartosiak-Jentys,

Kirstin L. Eley,

David J. Leak

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.07944-11

Subject(s) - geobacillus stearothermophilus , reporter gene , biology , gene , bioreporter , catechol , biochemistry , gene expression , microbiology and biotechnology , enzyme , thermophile

The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3-dioxygenase (C23O), catalyzes the formation of 2-hydroxymuconic semialdehyde, which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research