Rapid Genomic-Scale Analysis of Escherichia coli O104:H4 by Using High-Resolution Alternative Methods to Next-Generation Sequencing | Zendy

Scott A. Jackson | Zendy; Michael L. Kotewicz | Zendy; Isha R. Patel | Zendy; David W. Lacher | Zendy; Jayanthi Gangiredla | Zendy; Christopher A. Elkins | Zendy

Open Access

Rapid Genomic-Scale Analysis of Escherichia coli O104:H4 by Using High-Resolution Alternative Methods to Next-Generation Sequencing

Author(s) -

Scott A. Jackson,

Michael L. Kotewicz,

Isha R. Patel,

David W. Lacher,

Jayanthi Gangiredla,

Christopher A. Elkins

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.07464-11

Subject(s) - escherichia coli , biology , computational biology , dna sequencing , strain (injury) , genetics , serotype , genomics , genome , gene , microbiology and biotechnology , anatomy

Two technologies, involving DNA microarray and optical mapping, were used to quickly assess gene content and genomic architecture of recent emergent Escherichia coli O104:H4 and related strains. In real-time outbreak investigations, these technologies can provide congruent perspectives on strain, serotype, and pathotype relationships. Our data demonstrated clear discrimination between clinically, temporally, and geographically distinct O104:H4 isolates and rapid characterization of strain differences.

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