Open AccessCloning and Sequencing of Inulinase and β-Fructofuranosidase Genes of a Deep-Sea Microbulbifer Species and Properties of Recombinant Enzymes
Author(s) -
Tohru Kobayashi,
Kohsuke Uchimura,
Shigeru Deguchi,
Koki Horikoshi
Publication year - 2012
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.07442-11
Subject(s) - inulinase , cloning (programming) , recombinant dna , biology , gene , molecular cloning , enzyme , genetics , computational biology , microbiology and biotechnology , biochemistry , complementary dna , computer science , programming language
An inulinase-producingMicrobulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a β-fructofuranosidase. Both enzymes worked together to effectively degrade inulin.