Open AccessMarkerless Gene Deletion System for Sphingomonads
Author(s) -
Andreas Kaczmarczyk,
Julia A. Vorholt,
Anne FrancezCharlot
Publication year - 2012
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.07347-11
Subject(s) - genetics , streptomycin , gene , biology , allele , strain (injury) , antibiotics , anatomy
Here, we suggest that natural streptomycin resistance of many sphingomonads resides withinrpsL . We constructed a dominant, streptomycin-sensitiverpsL allele and demonstrated its use as a counterselection marker in several sphingomonads. AnrpsL -based markerless gene deletion system was developed and validated by deleting four genes inSphingomonas sp. strain Fr1.
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