Effectiveness of a Simplified Method for Isolation of Burkholderia pseudomallei from Soil | Zendy

Direk Limmathurotsakul | Zendy; Vanaporn Wuthiekanun | Zendy; Premjit Amornchai | Zendy; Gumphol Wongsuwan | Zendy; Nicholas Day | Zendy; Sharon J. Peacock | Zendy

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Effectiveness of a Simplified Method for Isolation of Burkholderia pseudomallei from Soil

Author(s) -

Direk Limmathurotsakul,

Vanaporn Wuthiekanun,

Premjit Amornchai,

Gumphol Wongsuwan,

Nicholas Day,

Sharon J. Peacock

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.07039-11

Subject(s) - burkholderia pseudomallei , melioidosis , isolation (microbiology) , burkholderia , microbiology and biotechnology , biology , bacteria , genetics

Detection of environmental Burkholderia pseudomallei indicates a risk for melioidosis and is important for the development of a global risk map. We describe a simple method for detecting B. pseudomallei using direct culture of soil in enrichment broth. This gives a rate of positivity comparable to that obtained with a standard method but is cheaper and labor saving.

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