Cultivated Single-Stranded DNA Phages That Infect Marine Bacteroidetes Prove Difficult To Detect with DNA-Binding Stains | Zendy

Karin Holmfeldt | Zendy; Duško Odić | Zendy; Matthew B. Sullivan | Zendy; Mathias Middelboe | Zendy; Lasse Riemann | Zendy

Open Access

Cultivated Single-Stranded DNA Phages That Infect Marine Bacteroidetes Prove Difficult To Detect with DNA-Binding Stains

Author(s) -

Karin Holmfeldt,

Duško Odić,

Matthew B. Sullivan,

Mathias Middelboe,

Lasse Riemann

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.06580-11

Subject(s) - bacteroidetes , dna , biology , bacterioplankton , dna sequencing , marine bacteriophage , bacteria , microbiology and biotechnology , genetics , 16s ribosomal rna , ecology , phytoplankton , nutrient

This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.

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