Identification and Characterization of a Xyloglucan-Specific Family 74 Glycosyl Hydrolase from Streptomyces coelicolor A3(2) | Zendy

Bolormaa Enkhbaatar | Zendy; Uyangaa Temuujin | Zendy; JuHyeon Lim | Zendy; WonJae Chi | Zendy; YongKeun Chang | Zendy; SoonKwang Hong | Zendy

Open Access

Identification and Characterization of a Xyloglucan-Specific Family 74 Glycosyl Hydrolase from Streptomyces coelicolor A3(2)

Author(s) -

Bolormaa Enkhbaatar,

Uyangaa Temuujin,

JuHyeon Lim,

WonJae Chi,

YongKeun Chang,

SoonKwang Hong

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.06482-11

Subject(s) - xyloglucan , streptomyces coelicolor , cellulase , glycosyl , hydrolase , biochemistry , glycoside hydrolase , streptomyces , actinomycetales , biology , chemistry , enzyme , gene , genetics , mutant , bacteria

Thesco6545 gene ofStreptomyces coelicolor A3(2) was nominated as a putative cellulase with 863 mature-form amino acids (90.58 kDa). We overexpressed and purified Sco6545 and demonstrated that the protein is not a cellulase but a xyloglucan-specific glycosyl hydrolase which cleaves xyloglucan at unbranched glucose residues.

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