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A multiplex methodology using two nuclease-resistant molecular beacons that target specific genomic regions of adenovirus 2 and echovirus 17 during simultaneous infection in A549 cells is presented. Using fluorescence microscopy, visualization of enteroviral and adenoviral replication was possible within 3 h postinfection.

Simultaneous Detection of Infectious Human Echoviruses and Adenoviruses by an In Situ Nuclease-Resistant Molecular Beacon-Based Assay