Simultaneous Detection of Infectious Human Echoviruses and Adenoviruses by an In Situ Nuclease-Resistant Molecular Beacon-Based Assay | Zendy

Daniela Dunams | Zendy; Payal Sarkar | Zendy; Wilfred Chen | Zendy; Marylynn V. Yates | Zendy

Open Access

Simultaneous Detection of Infectious Human Echoviruses and Adenoviruses by an In Situ Nuclease-Resistant Molecular Beacon-Based Assay

Author(s) -

Daniela Dunams,

Payal Sarkar,

Wilfred Chen,

Marylynn V. Yates

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.05937-11

Subject(s) - nuclease , virology , molecular beacon , biology , in situ , echovirus , microbiology and biotechnology , virus , chemistry , gene , genetics , enterovirus , organic chemistry , oligonucleotide

A multiplex methodology using two nuclease-resistant molecular beacons that target specific genomic regions of adenovirus 2 and echovirus 17 during simultaneous infection in A549 cells is presented. Using fluorescence microscopy, visualization of enteroviral and adenoviral replication was possible within 3 h postinfection.

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