Development of a Method To Produce Hemoglobin in a Bioreactor Culture of Escherichia coli BL21(DE3) Transformed with a Plasmid Containing Plesiomonas shigelloides Heme Transport Genes and Modified Human Hemoglobin Genes | Zendy

Bryan John Smith | Zendy; Paula Gutiérrez | Zendy; Elba Guerrero | Zendy; Charlotte Brewer | Zendy; Douglas P. Henderson | Zendy

Open Access

Development of a Method To Produce Hemoglobin in a Bioreactor Culture of Escherichia coli BL21(DE3) Transformed with a Plasmid Containing Plesiomonas shigelloides Heme Transport Genes and Modified Human Hemoglobin Genes

Author(s) -

Bryan John Smith,

Paula Gutiérrez,

Elba Guerrero,

Charlotte Brewer,

Douglas P. Henderson

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.05712-11

Subject(s) - escherichia coli , plesiomonas shigelloides , hemoglobin , plasmid , bioreactor , biology , heme , recombinant dna , microbiology and biotechnology , biochemistry , gene , bacteria , genetics , enzyme , botany

We describe a method for production of recombinant human hemoglobin by Escherichia coli grown in a bioreactor. E. coli BL21(DE3) transformed with a plasmid containing hemoglobin genes and Plesiomonas shigelloides heme transport genes reached a cell dry weight of 83.64 g/liter and produced 11.92 g/liter of hemoglobin in clarified lysates.

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