Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix | Zendy

Jette Kjeldgaard | Zendy; Sidsel Henriksen | Zendy; Marianne Thorup Cohn | Zendy; Søren Aabo | Zendy; Hanne Ingmer | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix

Author(s) -

Jette Kjeldgaard,

Sidsel Henriksen,

Marianne Thorup Cohn,

Søren Aabo,

Hanne Ingmer

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.05471-11

Subject(s) - gene expression , biology , computational biology , matrix (chemical analysis) , gene , genetics , microbiology and biotechnology , chemistry , chromatography

We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research