D1/D2 Domain of Large-Subunit Ribosomal DNA for Differentiation of Orpinomyces spp | Zendy

Sumit Singh Dagar | Zendy; Sanjay Kumar | Zendy; Priti Mudgil | Zendy; Prashant Singh | Zendy; Anil Kumar Puniya | Zendy

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D1/D2 Domain of Large-Subunit Ribosomal DNA for Differentiation of Orpinomyces spp

Author(s) -

Sumit Singh Dagar,

Sanjay Kumar,

Priti Mudgil,

Prashant Singh,

Anil Kumar Puniya

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.05441-11

Subject(s) - restriction fragment length polymorphism , ribosomal dna , biology , ribosomal rna , genetics , terminal restriction fragment length polymorphism , protein subunit , dna , restriction enzyme , microbiology and biotechnology , polymerase chain reaction , gene , phylogenetics

This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.

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