Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants | Zendy

Michael Tessaro | Zendy; Sameh S. M. Soliman | Zendy; Manish N. Raizada | Zendy

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Bacterial Whole-Cell Biosensor for Glutamine with Applications for Quantifying and Visualizing Glutamine in Plants

Author(s) -

Michael Tessaro,

Sameh S. M. Soliman,

Manish N. Raizada

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.05261-11

Subject(s) - glutamine , biosensor , escherichia coli , auxotrophy , biochemistry , biology , reporter gene , bacteria , chromatography , chemistry , gene , amino acid , gene expression , genetics

A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.

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