Use of Acid Treatment and a Selective Medium To Enhance the Recovery of Francisella tularensis from Water | Zendy

Ben W. Humrighouse | Zendy; Noreen J. Adcock | Zendy; Eugene W. Rice | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Use of Acid Treatment and a Selective Medium To Enhance the Recovery of Francisella tularensis from Water

Author(s) -

Ben W. Humrighouse,

Noreen J. Adcock,

Eugene W. Rice

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.05226-11

Subject(s) - francisella tularensis , fastidious organism , tularemia , microbiology and biotechnology , bacteria , biology , virulence , pathogen , isolation (microbiology) , agar , attenuated vaccine , agar plate , virology , biochemistry , genetics , gene

Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 15-min acid treatment procedure prior to culture on a selective agar was evaluated for recovery ofF. tularensis from seeded water samples. Mean levels of reduction of virulent strains ofF. tularensis subsp. holarctica and subsp.tularensis were less than 20% following acid treatment. The attenuated live vaccine strain (LVS) was less resistant to acid exposure. The acid treatment procedure coupled with plating on cystine heart agar with rabbit blood and antibiotics (CHARBab) allowed the isolation ofF. tularensis seeded into five natural water samples.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research