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Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiplealkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders byalkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA andalkB gene sequences were highly inconsistent.

Evaluating the Assignment of alkB Terminal Restriction Fragments and Sequence Types to Distinct Bacterial Taxa