Evaluating the Assignment of alkB Terminal Restriction Fragments and Sequence Types to Distinct Bacterial Taxa | Zendy

Julia Giebler | Zendy; Lukas Y. Wick | Zendy; Michael Schloter | Zendy; Hauke Harms | Zendy; Antonis Chatzinotas | Zendy

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Evaluating the Assignment of alkB Terminal Restriction Fragments and Sequence Types to Distinct Bacterial Taxa

Author(s) -

Julia Giebler,

Lukas Y. Wick,

Michael Schloter,

Hauke Harms,

Antonis Chatzinotas

Publication year - 2013

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.04028-12

Subject(s) - alkb , biology , genetics , terminal restriction fragment length polymorphism , sequence (biology) , terminal (telecommunication) , computational biology , restriction fragment length polymorphism , gene , escherichia coli , computer science , polymerase chain reaction , telecommunications

Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiplealkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders byalkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA andalkB gene sequences were highly inconsistent.

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